a) Temperature gradient PCR of Rv0148 (52°C-56°C for Lane 1-5). (b)
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![](https://www.researchgate.net/profile/Divakar-Sharma/publication/281585159/figure/fig1/AS:369865689976833@1465194260423/Fig-1-a-Temperature-gradient-PCR-of-Rv0148-52C-56C-for-Lane-1-5-b.png)
![](https://www.researchgate.net/profile/Divakar-Sharma/publication/281585159/figure/fig1/AS:369865689976833@1465194260423/Fig-1-a-Temperature-gradient-PCR-of-Rv0148-52C-56C-for-Lane-1-5-b.png)
a) Temperature gradient PCR of Rv0148 (52°C-56°C for Lane 1-5). (b
![](https://www.researchgate.net/publication/275717545/figure/tbl1/AS:11431281220192610@1706278232533/Numbers-of-chicken-meat-samples-by-type-of-resistant-E-coli-isolate.jpeg)
Numbers of chicken meat samples by type of resistant E. coli
![](https://www.researchgate.net/profile/Tonmoy-Chowdhury-3/post/How_to_fix_to_many_non_specific_band_in_PCR/attachment/637770a828b5df6ceff1c301/AS%3A11431281098083961%401668772008246/image/IMG_20221118_174056.jpg)
155 questions with answers in GRADIENT PCR
![](https://www.researchgate.net/profile/Reyhaneh-Ebrahimi-Khaksefidi/post/Genotyping_issue_for_edited_plants_using_CRISPR_Cas9_system/attachment/62c67d170c295f1f9aca4fe1/AS%3A1175092612603905%401657175319813/image/Q2.jpg)
155 questions with answers in GRADIENT PCR
![](https://www.researchgate.net/publication/317822405/figure/fig4/AS:779394155028514@1562833455403/a-Amplification-curves-of-gradient-PCR-NTC-denotes-no-template-control-b-Melt-curve.gif)
a Amplification curves of gradient PCR. NTC denotes no template
![](https://www.researchgate.net/profile/Anam-Farooqui-2/post/Why_am_i_getting_multiple_bands_in_semi-quantitative_PCR_of_my_TNF_alpha_and_akt1_gene/attachment/62bc4ce2c223cb5aef8e663e/AS%3A1172292067426304%401656507617825/image/1656440394047+edit.jpg)
155 questions with answers in GRADIENT PCR
![](https://www.bio-rad.com/webroot/web/images/lsr/solutions/technologies/gene_expression/pcr/technology_detail/gradient-optimization-of-a-qPCR-assay.jpg)
qPCR Assay Design and Optimization
![](https://i.ytimg.com/vi/1vqNZ-H7Pq0/hq720.jpg?sqp=-oaymwEhCK4FEIIDSFryq4qpAxMIARUAAAAAGAElAADIQj0AgKJD&rs=AOn4CLBG7xDSqgwNZB2zJ9lCbE3vx0-uQQ)
Gradient PCR
![](https://insitutech.files.wordpress.com/2016/06/img_0343.jpg?w=1200)
Gradient PCR using NEB Polymerase – insitutech
![](https://biolabscientific.com/content/products-images/384-Well-Gradient-Thermal-Cycler-BTHC-115-384-well-In-situ-Plate-0C-100C-Analytical-Laboratories-Molecular-biology-Gradient-PCR-Machine-m1-Biolab.jpg)
384 Well Gradient Thermal Cycler BTHC-115
![](https://s3.us-west-1.amazonaws.com/resource.bioneer.us/juan2/us/page/4014/images/ThermalGradient1.png)
Bioneer Inc
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